Motility-based label-free detection of parasites in bodily fluids using holographic speckle analysis and deep learning

Yibo Zhang; Hatice Ceylan Koydemir; Michelle M. Shimogawa; Sener Yalcin; Alexander Guziak; Tairan Liu; Ilker Oguz; Yujia Huang; Bijie Bai; Yilin Luo; Yi Luo; Zhensong Wei; Hongda Wang; Vittorio Bianco; Bohan Zhang; Rohan Nadkarni; Kent Hill; Aydogan Ozcan

doi:10.1038/s41377-018-0110-1

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Motility-based label-free detection of parasites in bodily fluids using holographic speckle analysis and deep learning

Original Articles

- Motility-based label-free detection of parasites in bodily fluids using holographic speckle analysis and deep learning
- Motility-based label-free detection of parasites in bodily fluids using holographic speckle analysis and deep learning
- 光：科学与应用 2018年7卷第6期页码：1028-1045
- Affiliations：
  
  1.Electrical and Computer Engineering Department, University of California, Los Angeles, CA, 90095, USA
  2.Bioengineering Department, University of California, Los Angeles, CA, 90095, USA
  3.California NanoSystems Institute, University of California, Los Angeles, CA, 90095, USA
  4.Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA, 90095, USA
  5.Department of Physics and Astronomy, University of California, Los Angeles, CA, 90095, USA
  6.Molecular Biology Institute, University of California, Los Angeles, CA, 90095, USA
  7.Department of Surgery, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA
- Author bio：
  
  Aydogan Ozcan (ozcan@ucla.edu)
- Funds：
- DOI：10.1038/s41377-018-0110-1
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Yibo Zhang, Hatice Ceylan Koydemir, Michelle M. Shimogawa, 等. Motility-based label-free detection of parasites in bodily fluids using holographic speckle analysis and deep learning[J]. 光：科学与应用, 2018,7(6):1028-1045.

Zhang, Y. B. et al. Motility-based label-free detection of parasites in bodily fluids using holographic speckle analysis and deep learning. Light: Science & Applications, 7, 1028-1045 (2018).
Yibo Zhang, Hatice Ceylan Koydemir, Michelle M. Shimogawa, 等. Motility-based label-free detection of parasites in bodily fluids using holographic speckle analysis and deep learning[J]. 光：科学与应用, 2018,7(6):1028-1045. DOI： 10.1038/s41377-018-0110-1.

Zhang, Y. B. et al. Motility-based label-free detection of parasites in bodily fluids using holographic speckle analysis and deep learning. Light: Science & Applications, 7, 1028-1045 (2018). DOI： 10.1038/s41377-018-0110-1.

摘要

Abstract

Parasitic infections constitute a major global public health issue. Existing screening methods that are based on manual microscopic examination often struggle to provide sufficient volumetric throughput and sensitivity to facilitate early diagnosis. Here, we demonstrate a motility-based label-free computational imaging platform to rapidly detect motile parasites in optically dense bodily fluids by utilizing the locomotion of the parasites as a specific biomarker and endogenous contrast mechanism. Based on this principle, a cost-effective and mobile instrument, which rapidly screens ~3.2 mL of fluid sample in three dimensions, was built to automatically detect and count motile microorganisms using their holographic time-lapse speckle patterns. We demonstrate the capabilities of our platform by detecting trypanosomes, which are motile protozoan parasites, with various species that cause deadly diseases affecting millions of people worldwide. Using a holographic speckle analysis algorithm combined with deep learning-based classification, we demonstrate sensitive and label-free detection of trypanosomes within spiked whole blood and artificial cerebrospinal fluid (CSF) samples, achieving a limit of detection of ten trypanosomes per mL of whole blood (~five-fold better than the current state-of-the-art parasitological method) and three trypanosomes per mL of CSF. We further demonstrate that this platform can be applied to detect other motile parasites by imaging,Trichomonas vaginalis, the causative agent of trichomoniasis, which affects 275 million people worldwide. With its cost-effective, portable design and rapid screening time, this unique platform has the potential to be applied for sensitive and timely diagnosis of neglected tropical diseases caused by motile parasites and other parasitic infections in resource-limited regions.

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references

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