Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber

Sebastian A. Vasquez-Lopez; Raphaël Turcotte; Vadim Koren; Martin Plöschner; Zahid Padamsey; Martin J. Booth; Tomáš Čižmár; Nigel J. Emptage

doi:10.1038/s41377-018-0111-0

Go to Nature Website

Your Location：

Home >

Browse articles >

Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber

Letter

- Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber
- Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber
- 光：科学与应用 2018年7卷第6期页码：686-691
- Affiliations：
  
  1.Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK
  2.Department of Engineering Science, University of Oxford, Parks Road, Oxford, OX1 3PJ, UK
  3.School of Engineering, Physics and Mathematics, College of Art, Science & Engineering, University of Dundee, Nethergate, Dundee, DD1 4HN, Scotland, UK
  4.Institute of Scientific Instruments of the CAS, Královopolská 147, 612 64, Brno, Czech Republic
- Author bio：
  
  Nigel J. Emptage (nigel.emptage@pharm.ox.ac.uk)
- Funds：
- DOI：10.1038/s41377-018-0111-0
  中图分类号：
Scan for full text
Sebastian A. Vasquez-Lopez, Raphaël Turcotte, Vadim Koren, 等. Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber[J]. 光：科学与应用, 2018,7(6):686-691.

Vasquez-Lopez, S. A. et al. Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber.Light: Science & Applications, 7, 686-691 (2018).
Sebastian A. Vasquez-Lopez, Raphaël Turcotte, Vadim Koren, 等. Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber[J]. 光：科学与应用, 2018,7(6):686-691. DOI： 10.1038/s41377-018-0111-0.

Vasquez-Lopez, S. A. et al. Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber.Light: Science & Applications, 7, 686-691 (2018). DOI： 10.1038/s41377-018-0111-0.

摘要

Abstract

Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system,1,1,-,4,4,1-4,. Advances in wavefront-shaping methods and computational power have recently allowed for a novel approach to high-resolution imaging, utilizing deterministic light propagation through optically complex media and, of particular importance for this work, multimode optical fibers (MMFs),5,5,-,7,7,5-7,. We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications. The volume of tissue lesion was reduced by more than 100-fold, while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-μm-core MMF. Here, we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures, dendrites and synaptic specializations, in deep-brain regions of living mice, as well as monitored stimulus-driven functional Ca,2+, responses. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo.

关键词

Keywords

references

Ji, N. The practical and fundamental limits of optical imaging in mammalian brains. Neuron 83, 1242–1245 (2014).

Andersen, P., Morris, R., Amaral, D., Bliss, T. & O' Keefe, J. The Hippocampus Book. (Oxford University Press, Oxford, New York, 2007).

Jones, E. G. The Thalamus. (Cambridge University Press, Cambridge, 2007).

Misgeld, T. & Kerschensteiner, M. In vivo imaging of the diseased nervous system. Nat. Rev. Neurosci. 7, 449–463 (2006).

Čižmár, T. & Dholakia, K. Shaping the light transmission through a multimode optical fibre: complex transformation analysis and applications in biophotonics. Opt. Express 19, 18871–18884 (2011).

Čižmár, T. & Dholakia, K. Exploiting multimode waveguides for pure fibre-based imaging. Nat. Commun. 3, 1027 (2012).

Plöschner, M. & Čižmár, T. Compact multimode fiber beam-shaping system based on GPU accelerated digital holography. Opt. Lett. 40, 197–200 (2015).

Dombeck, D. A., Harvey, C. D., Tian, L., Looger, L. L. & Tank, D. W. Functional imaging of hippocampal place cells at cellular resolution during virtual navigation. Nat. Neurosci. 13, 1433–1440 (2010).

Szabo, V., Ventalon, C., De Sars, V., Bradley, J. & Emiliani, V. Spatially selective holographic Photoactivation and functional fluorescence imaging in freely behaving mice with a fiberscope. Neuron 84, 1157–1169 (2014).

Barretto, R. P. J. et al. Time-lapse imaging of disease progression in deep brain areas using fluorescence microendoscopy. Nat. Med. 17, 223–228 (2011).

Resendez, S. L. et al. Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses. Nat. Protoc. 11, 566–597 (2016).

Bocarsly, M. E. et al. Minimally invasive microendoscopy system for in vivo functional imaging of deep nuclei in the mouse brain. Biomed. Opt. Express 6, 4546–4556 (2015).

Xu, H. T., Pan, F., Yang, G. & Gan, W. B. Choice of cranial window type for in vivo imaging affects dendritic spine turnover in the cortex. Nat. Neurosci. 10, 549–551 (2007).

Moshayedi, P. et al. The relationship between glial cell mechanosensitivity and foreign body reactions in the central nervous system. Biomaterials 35, 3919–3925 (2014).

Popoff, S. M. et al. Measuring the transmission matrix in optics: an approach to the study and control of light propagation in disordered media. Phys. Rev. Lett. 104, 100601 (2010).

Mahalati, R. N., Gu, R. Y. & Kahn, J. M. Resolution limits for imaging through multi-mode fiber. Opt. Express 21, 1656–1668 (2013).

Kim, G. et al. Deep-brain imaging via epi-fluorescence computational cannula microscopy. Sci. Rep. 7, 44791 (2017).

Feng, G. et al. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28, 41–51 (2000).

Crowe, S. E. & Ellis-Davies, G. C. R. Longitudinal in vivo two-photon fluorescence imaging. J. Comp. Neurol. 522, 1708–1727 (2014).

Ohayon, S., Caravaca-Aguirre, A., Piestun, R. & DiCarlo, J. J. Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging. Biomed. Opt. Express 9, 1492–1509 (2018).

Plöschner, M., Straka, B., Dholakia, K. & Čižmár, T. GPU accelerated toolbox for real-time beam-shaping in multimode fibres. Opt. Express 22, 2933–2947 (2014).

Conkey, D. B., Caravaca-Aguirre, A. M. & Piestun, R. High-speed scattering medium characterization with application to focusing light through turbid media. Opt. Express 20, 1733–1740 (2012).

Mitchell, K. J., Turtaev, S., Padgett, M. J., Čižmár, T. & Phillips, D. B. High-speed spatial control of the intensity, phase and polarisation of vector beams using a digital micro-mirror device. Opt. Express 24, 29269–29282 (2016).

Turtaev, S. et al. Comparison of nematic liquid-crystal and DMD based spatial light modulation in complex photonics. Opt. Express 25, 29874–29884 (2017).

Gigan, S. Optical microscopy aims deep. Nat. Photon 11, 14–16 (2017).

Loterie, D. et al. Digital confocal microscopy through a multimode fiber. Opt. Express 23, 23845–23858 (2015).

Morales-Delgado, E. E., Farahi, S., Papadopoulos, I. N., Psaltis, D. & Moser, C. Delivery of focused short pulses through a multimode fiber. Opt. Express 23, 9109–9120 (2015).

Sahl, S. J., Hell, S. W. & Jakobs, S. Fluorescence nanoscopy in cell biology. Nat. Rev. Mol. Cell Biol. 18, 685–701 (2017).

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

暂无数据