PhaseStain: the digital staining of label-free quantitative phase microscopy images using deep learning

Yair Rivenson; Tairan Liu; Zhensong Wei; Yibo Zhang; Kevin de Haan; Aydogan Ozcan

doi:10.1038/s41377-019-0129-y

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PhaseStain: the digital staining of label-free quantitative phase microscopy images using deep learning

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- PhaseStain: the digital staining of label-free quantitative phase microscopy images using deep learning
- PhaseStain: the digital staining of label-free quantitative phase microscopy images using deep learning
- LSA 2019年8卷第1期页码：172-182
- Affiliations：
  
  1.Electrical and Computer Engineering Department, University of California, Los Angeles, CA, 90095, USA
  2.Bioengineering Department, University of California, Los Angeles, CA, 90095, USA
  3.California NanoSystems Institute (CNSI), University of California, Los Angeles, CA, 90095, USA
  4.Department of Surgery, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA
- Author bio：
  
  Aydogan Ozcan (ozcan@ucla.edu)
- Funds：
- DOI：10.1038/s41377-019-0129-y
  中图分类号：
- 纸质出版日期：2019，
  
  网络出版日期：2019-02-06，
  
  收稿日期：2018-07-23，
  
  修回日期：2019-01-05，
  
  录用日期：2019-01-11
- Accepted：
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PhaseStain: the digital staining of label-free quantitative phase microscopy images using deep learning[J]. LSA, 2019,8(1):172-182.

Rivenson, Y. et al. PhaseStain: the digital staining of label-free quantitative phase microscopy images using deep learning. Light: Science & Applications, 8, 172-182 (2019).
PhaseStain: the digital staining of label-free quantitative phase microscopy images using deep learning[J]. LSA, 2019,8(1):172-182. DOI： 10.1038/s41377-019-0129-y.

Rivenson, Y. et al. PhaseStain: the digital staining of label-free quantitative phase microscopy images using deep learning. Light: Science & Applications, 8, 172-182 (2019). DOI： 10.1038/s41377-019-0129-y.

摘要

Abstract

Using a deep neural network

we demonstrate a digital staining technique

which we term PhaseStain

to transform the quantitative phase images (QPI) of label-free tissue sections into images that are equivalent to the brightfield microscopy images of the same samples that are histologically stained. Through pairs of image data (QPI and the corresponding brightfield images

acquired after staining)

we train a generative adversarial network and demonstrate the effectiveness of this virtual-staining approach using sections of human skin

kidney

and liver tissue

matching the brightfield microscopy images of the same samples stained with Hematoxylin and Eosin

Jones' stain

and Masson's trichrome stain

respectively. This digital-staining framework may further strengthen various uses of label-free QPI techniques in pathology applications and biomedical research in general

by eliminating the need for histological staining

reducing sample preparation related costs and saving time. Our results provide a powerful example of some of the unique opportunities created by data-driven image transformations enabled by deep learning.

关键词

Keywords

references

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