MATRIEX imaging: multiarea two-photon real-time in vivo explorer

Mengke Yang; Zhenqiao Zhou; Jianxiong Zhang; Shanshan Jia; Tong Li; Jiangheng Guan; Xiang Liao; Bing Leng; Jing Lyu; Kuan Zhang; Min Li; Yan Gong; Zhiming Zhu; Junan Yan; Yi Zhou; Jian K Liu; Zsuzsanna Varga; Arthur Konnerth; Yuguo Tang; Jinsong Gao; Xiaowei Chen; Hongbo Jia

doi:10.1038/s41377-019-0219-x

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MATRIEX imaging: multiarea two-photon real-time in vivo explorer

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- MATRIEX imaging: multiarea two-photon real-time in vivo explorer
- MATRIEX imaging: multiarea two-photon real-time in vivo explorer
- LSA 2019年8卷第6期页码：1073-1083
- Affiliations：
  
  1.Key Laboratory of Optical System Advanced Manufacturing Technology, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China
  2.Graduate School, University of the Chinese Academy of Sciences, Beijing 100039, China
  3.Brain Research Instrument Innovation Center, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China
  4.Brain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing 400038, China
  5.Center for Neurointelligence, Chongqing University, Chongqing 401331, China
  6.Center for Hypertension and Metabolic Diseases, Daping Hospital, Chongqing 400042, China
  7.Advanced Institute of Brain and Intelligence, Guangxi University, Nanning 530005, China
  8.Centre for Systems Neuroscience, Department of Neuroscience, Psychology and Behaviour, University of Leicester, Leicester, UK
  9.Institute of Neuroscience, Technical University Munich, 80802 Munich, Germany
- Author bio：
  
  Zhenqiao Zhou (zhouzq@sibet.ac.cn)
  Yuguo Tang (tangyg@sibet.ac.cn)
  Jinsong Gao (gaojs@ciomp.ac.cn)
- Funds：
- DOI：10.1038/s41377-019-0219-x
  中图分类号：
- 纸质出版日期：2019，
  
  网络出版日期：2019-11-28，
  
  收稿日期：2019-07-16，
  
  修回日期：2019-10-31，
  
  录用日期：2019-11-05
- Accepted：
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MATRIEX imaging: multiarea two-photon real-time in vivo explorer[J]. LSA, 2019,8(6):1073-1083.

Yang, M. K. et al. MATRIEX imaging: multiarea two-photon real-time in vivo explorer. Light: Science & Applications, 8, 1073-1083 (2019).
MATRIEX imaging: multiarea two-photon real-time in vivo explorer[J]. LSA, 2019,8(6):1073-1083. DOI： 10.1038/s41377-019-0219-x.

Yang, M. K. et al. MATRIEX imaging: multiarea two-photon real-time in vivo explorer. Light: Science & Applications, 8, 1073-1083 (2019). DOI： 10.1038/s41377-019-0219-x.

摘要

Abstract

Two-photon laser scanning microscopy has been extensively applied to study in vivo neuronal activity at cellular and subcellular resolutions in mammalian brains. However

the extent of such studies is typically confined to a single functional region of the brain. Here

we demonstrate a novel technique

termed the multiarea two-photon real-time in vivo explorer (MATRIEX)

that allows the user to target multiple functional brain regions distributed within a zone of up to 12 mm in diameter

each with a field of view (FOV) of ~200 ǌm in diameter

thus performing two-photon Ca

imaging with single-cell resolution in all of the regions simultaneously. For example

we demonstrate real-time functional imaging of single-neuron activities in the primary visual cortex

primary motor cortex and hippocampal CA1 region of mice in both anesthetized and awake states. A unique advantage of the MATRIEX technique is the configuration of multiple microscopic FOVs that are distributed in three-dimensional space over macroscopic distances (

1 mm) both laterally and axially but that are imaged by a single conventional laser scanning device. In particular

the MATRIEX technique can be effectively implemented as an add-on optical module for an existing conventional single-beam-scanning two-photon microscope without requiring any modification to the microscope itself. Thus

the MATRIEX technique can be readily applied to substantially facilitate the exploration of multiarea neuronal activity in vivo for studies of brain-wide neural circuit function with single-cell resolution.

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references

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