Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids

Jules Scholler; Kassandra Groux; Olivier Goureau; José-Alain Sahel; Mathias Fink; Sacha Reichman; Claude Boccara; Kate Grieve

doi:10.1038/s41377-020-00375-8

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Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids

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- Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids
- Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids
- LSA 2020年9卷第5期页码：1306-1314
- Affiliations：
  
  1.Institut Langevin, ESPCI Paris, PSL University, CNRS, 10 rue Vauquelin, Paris, France
  2.Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012 Paris, France
  3.Quinze-Vingts National Eye Hospital, 28 Rue de Charenton, Paris 75012, France
  4.Fondation Ophtalmologique Rothschild, F-75019 Paris, France
  5.Department of Ophthalmology, The University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, United States
- Author bio：
  
  Kate Grieve (kategrieve@gmail.com)
- Funds：
- DOI：10.1038/s41377-020-00375-8
  中图分类号：
- 纸质出版日期：2020，
  
  网络出版日期：2020-08-17，
  
  收稿日期：2019-12-09，
  
  修回日期：2020-06-19，
  
  录用日期：2020-07-27
- Accepted：
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Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids[J]. LSA, 2020,9(5):1306-1314.

Scholler, J. et al. Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids. Light: Science & Applications, 9, 1306-1314 (2020).
Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids[J]. LSA, 2020,9(5):1306-1314. DOI： 10.1038/s41377-020-00375-8.

Scholler, J. et al. Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids. Light: Science & Applications, 9, 1306-1314 (2020). DOI： 10.1038/s41377-020-00375-8.

摘要

Abstract

Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information. We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell-derived retinal organoids. Coloured images with an endogenous contrast linked to organelle motility are generated

with submicrometre spatial resolution and millisecond temporal resolution

creating a way to identify specific cell types in living tissue via their function.

关键词

Keywords

references

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