Converting lateral scanning into axial focusing to speed up three-dimensional microscopy

Tonmoy Chakraborty; Bingying Chen; Stephan Daetwyler; Bo-Jui Chang; Oliver Vanderpoorten; Etai Sapoznik; Clemens F. Kaminski; Tuomas P. J. Knowles; Kevin M. Dean; Reto Fiolka

doi:10.1038/s41377-020-00401-9

Go to Nature Website

Your Location：

Home >

Browse articles >

Converting lateral scanning into axial focusing to speed up three-dimensional microscopy

Original Articles

- Converting lateral scanning into axial focusing to speed up three-dimensional microscopy
- Converting lateral scanning into axial focusing to speed up three-dimensional microscopy
- LSA 2020年9卷第5期页码：1587-1598
- Affiliations：
  
  1.Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX, USA
  2.Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM, USA
  3.Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge CB3 0AS, UK
  4.Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
  5.Lyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, TX, USA
- Author bio：
  
  Reto Fiolka (Reto.Fiolka@utsouthwestern.edu)
- Funds：
- DOI：10.1038/s41377-020-00401-9
  中图分类号：
- 纸质出版日期：2020，
  
  网络出版日期：2020-09-18，
  
  收稿日期：2020-03-18，
  
  修回日期：2020-08-25，
  
  录用日期：2020-09-02
- Accepted：
Scan QR Code
Converting lateral scanning into axial focusing to speed up three-dimensional microscopy[J]. LSA, 2020,9(5):1587-1598.

Chakraborty, T. et al. Converting lateral scanning into axial focusing to speed up three-dimensional microscopy. Light: Science & Applications, 9, 1587-1598 (2020).
Converting lateral scanning into axial focusing to speed up three-dimensional microscopy[J]. LSA, 2020,9(5):1587-1598. DOI： 10.1038/s41377-020-00401-9.

Chakraborty, T. et al. Converting lateral scanning into axial focusing to speed up three-dimensional microscopy. Light: Science & Applications, 9, 1587-1598 (2020). DOI： 10.1038/s41377-020-00401-9.

摘要

Abstract

In optical microscopy

the slow axial scanning rate of the objective or the sample has traditionally limited the speed of volumetric imaging. Recently

by conjugating either a movable mirror to the image plane in a remote-focusing geometry or an electrically tuneable lens (ETL) to the back focal plane

rapid axial scanning has been achieved. However

mechanical actuation of a mirror limits the axial scanning rate (usually only 10–100 Hz for piezoelectric or voice coil-based actuators)

while ETLs introduce spherical and higher-order aberrations that prevent high-resolution imaging. In an effort to overcome these limitations

we introduce a novel optical design that transforms a lateral-scan motion into a spherical aberration-free axial scan that can be used for high-resolution imaging. Using a galvanometric mirror

we scan a laser beam laterally in a remote-focusing arm

which is then back-reflected from different heights of a mirror in the image space. We characterize the optical performance of this remote-focusing technique and use it to accelerate axially swept light-sheet microscopy by an order of magnitude

allowing the quantification of rapid vesicular dynamics in three dimensions. We also demonstrate resonant remote focusing at 12 kHz with a two-photon raster-scanning microscope

which allows rapid imaging of brain tissues and zebrafish cardiac dynamics with diffraction-limited resolution.

关键词

Keywords

references

Gong, Y. Y. et al. High-speed recording of neural spikes in awake mice and flies with a fluorescent voltage sensor.Science350, 1361–1366 (2015)..

Botcherby, E. J. et al. Aberration-free optical refocusing in high numerical aperture microscopy.Opt. Lett.32, 2007–2009 (2007)..

Botcherby, E. J. et al. Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates.Proc. Natl Acad. Sci. USA109, 2919–2924 (2012)..

Mermillod-Blondin, A., McLeod, E.&Arnold, C. B. High-speed varifocal imaging with a tunable acoustic gradient index of refraction lens.Opt. Lett.33, 2146–2148 (2008)..

Duocastella, M., Sun, B.&Arnold, C. B. Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics.J. Biomed. Opt.17, 050505 (2012)..

Berge, B.&Peseux, J. Variable focal lens controlled by an external voltage: an application of electrowetting.Eur. Phys. J. E3, 159–163 (2000)..

Žurauskas, M. et al. Rapid adaptive remote focusing microscope for sensing of volumetric neural activity.Biomed. Opt. Express8, 4369–4379 (2017)..

Fahrbach, F. O. et al. Rapid 3D light-sheet microscopy with a tunable lens.Opt. Express21, 21010–21026 (2013)..

Ozbay, B. N. et al. Miniaturized fiber-coupled confocal fluorescence microscope with an electrowetting variable focus lens using no moving parts.Opt. Lett.40, 2553–2556 (2015)..

Stirman, J. N. et al. Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain.Nat. Biotechnol.34, 857–862 (2016)..

Cheng, A. et al. Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing.Nat. Methods8, 139–142 (2011)..

Han, S. T., Yang, W. J.&Yuste, R. Two-color volumetric imaging of neuronal activity of cortical columns.Cell Rep.27, 2229–2240.e4 (2019)..

Weisenburger, S. et al. Volumetric Ca2+imaging in the mouse brain using hybrid multiplexed sculpted light microscopy.Cell177, 1050–1066.e14 (2019)..

Beaulieu, D. R. et al. Simultaneous multiplane imaging with reverberation two-photon microscopy.Nat. Methods17, 283–286 (2020)..

Lu, R. W. et al. Video-rate volumetric functional imaging of the brain at synaptic resolution.Nat. Neurosci.20, 620–628 (2017)..

Song, A. et al. Volumetric two-photon imaging of neurons using stereoscopy (vTwINS).Nat. Methods14, 420–426 (2017)..

Wu, J. L. et al. Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo.Nat. Methods17, 287–290 (2020)..

Schneider, J. et al. Ultrafast, temporally stochastic STED nanoscopy of millisecond dynamics.Nat. Methods12, 827–830 (2015)..

Salomé, R. et al. Ultrafast random-access scanning in two-photon microscopy using acousto-optic deflectors.J. Neurosci. Methods154, 161–174 (2006)..

Chakraborty, T. et al. Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution.Nat. Methods16, 1109–1113 (2019)..

Dean, K. M. et al. Deconvolution-free subcellular imaging with axially swept light sheet microscopy.Biophysical J.108, 2807–2815 (2015)..

Delarue, M. et al. mTORC1 controls phase separation and the biophysical properties of the cytoplasm by tuning crowding.Cell174, 338–349.e20 (2018)..

Nguyen, C. T. et al. Zebrafish as a model for cardiovascular development and disease.Drug Discov. Today5, 135–140 (2008)..

Weber, M.&Huisken, J. In vivo imaging of cardiac development and function in zebrafish using light sheet microscopy.Swiss Med. Wkly.145, w14227 (2015)..

Jin, S. W. Cellular and molecular analyses of vascular tube and lumen formation in zebrafish.Development132, 5199–5209 (2005)..

Voigt, F. F. et al. The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue.Nat. Methods16, 1105–1108 (2019)..

Kim, D. Y. et al. Lissajous scanning two-photon endomicroscope forin vivotissue imaging.Sci. Rep.9, 3560 (2019)..

Challa, P. K. et al. Microfluidic devices fabricated using fast wafer-scale LED-lithography patterning.Biomicrofluidics11, 014113 (2017)..

Gan, Z. et al. Vimentin intermediate filaments template microtubule networks to enhance persistence in cell polarity and directed migration.Cell Syst.3, 252–263.e8 (2016)..

Jaqaman, K. et al. Robust single-particle tracking in live-cell time-lapse sequences.Nat. Methods5, 695–702 (2008)..

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

暂无数据