Superchiral near fields detect virus structure

Tarun Kakkar; Chantal Keijzer; Marion Rodier; Tatyana Bukharova; Michael Taliansky; Andrew J. Love; Joel J. Milner; Affar S. Karimullah; Laurence D. Barron; Nikolaj Gadegaard; Adrian J. Lapthorn; Malcolm Kadodwala

doi:10.1038/s41377-020-00433-1

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Superchiral near fields detect virus structure

Original Articles

- Superchiral near fields detect virus structure
- Superchiral near fields detect virus structure
- LSA 2020年9卷第6期页码：1983-1992
- Affiliations：
  
  1.School of Chemistry, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK
  2.Institute of Molecular, Cell and Systems Biology and School of Life Sciences, University of Glasgow, G12 8QQ Glasgow, UK
  3.James Hutton Inst, Cell & Mol Sci, Dundee DD2 5DA, UK
  4.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow 117997, Russia
  5.School of Engineering, Rankine Building, University of Glasgow, Glasgow G12 8LT, UK
- Author bio：
  
  Tarun Kakkar (tarun.kakkar2@gmail.com)
  Chantal Keijzer (keijzerchantal@gmail.com)
  Malcolm Kadodwala (malcolm.kadodwala@glasgow.ac.uk)
- Funds：
- DOI：10.1038/s41377-020-00433-1
  中图分类号：
- 纸质出版日期：2020，
  
  网络出版日期：2020-12-01，
  
  收稿日期：2020-05-18，
  
  修回日期：2020-10-28，
  
  录用日期：2020-11-11
- Accepted：
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Superchiral near fields detect virus structure[J]. LSA, 2020,9(6):1983-1992.

Kakkar, T. et al. Superchiral near fields detect virus structure. Light: Science & Applications, 9, 1983-1992 (2020).
Superchiral near fields detect virus structure[J]. LSA, 2020,9(6):1983-1992. DOI： 10.1038/s41377-020-00433-1.

Kakkar, T. et al. Superchiral near fields detect virus structure. Light: Science & Applications, 9, 1983-1992 (2020). DOI： 10.1038/s41377-020-00433-1.

摘要

Abstract

Optical spectroscopy can be used to quickly characterise the structural properties of individual molecules. However

it cannot be applied to biological assemblies because light is generally blind to the spatial distribution of the component molecules. This insensitivity arises from the mismatch in length scales between the assemblies (a few tens of nm) and the wavelength of light required to excite chromophores (≥150 nm). Consequently

with conventional spectroscopy

ordered assemblies

such as the icosahedral capsids of viruses

appear to be indistinguishable isotropic spherical objects. This limits potential routes to rapid high-throughput portable detection appropriate for point-of-care diagnostics. Here

we demonstrate that chiral electromagnetic (EM) near fields

which have both enhanced chiral asymmetry (referred to as superchirality) and subwavelength spatial localisation (~10 nm)

can detect the icosahedral structure of virus capsids. Thus

they can detect both the presence and relative orientation of a bound virus capsid. To illustrate the potential uses of the exquisite structural sensitivity of subwavelength superchiral fields

we have used them to successfully detect virus particles in the complex milieu of blood serum.

关键词

Keywords

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