New solution for fast axial scanning in fluorescence microscopy

Weijian Zong

doi:10.1038/s41377-020-00442-0

Go to Nature Website

Your Location：

Home >

Browse articles >

New solution for fast axial scanning in fluorescence microscopy

News and Views

- New solution for fast axial scanning in fluorescence microscopy
- New solution for fast axial scanning in fluorescence microscopy
- LSA 2020年9卷第6期页码：1694-1695
- Affiliations：
  
  Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, Norway
- Author bio：
  
  Weijian Zong (weijian.zong@ntnu.no)
- Funds：
- DOI：10.1038/s41377-020-00442-0
  中图分类号：
- 纸质出版日期：2020，
  
  网络出版日期：2020-12-14，
- Accepted：
Scan QR Code
New solution for fast axial scanning in fluorescence microscopy[J]. LSA, 2020,9(6):1694-1695.

Zong, W. J. New solution for fast axial scanning in fluorescence microscopy. Light: Science & Applications, 9, 1694-1695 (2020).
New solution for fast axial scanning in fluorescence microscopy[J]. LSA, 2020,9(6):1694-1695. DOI： 10.1038/s41377-020-00442-0.

Zong, W. J. New solution for fast axial scanning in fluorescence microscopy. Light: Science & Applications, 9, 1694-1695 (2020). DOI： 10.1038/s41377-020-00442-0.

摘要

Abstract

A novel technique based on the remote-focusing concept

using a galvanometer scanner combined with a self-fabricated "step mirror" or "tilted mirror" to transform fast lateral scanning into axial scanning

was reported as a new solution for fast

subcellular

3D fluorescence imaging.

关键词

Keywords

references

Liu, T. L. et al. Observing the cell in its native state: imaging subcellular dynamics in multicellular organisms.Science360, eaaq1392 (2018)..

Fahrbach, F. O. et al. Rapid 3D light-sheet microscopy with a tunable lens.Opt. Express21, 21010-21026 (2013)..

Grewe, B. F. et al. Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens.Biomed. Opt. Express2, 2035-2046 (2011)..

Botcherby, E. J. et al. Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates.Proc. Natl Acad. Sci. USA109, 2919-2924 (2012)..

Wu, J. L. et al. Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo.Nat. Methods17, 287-290 (2020)..

Beaulieu, D. R. et al. Simultaneous multiplane imaging with reverberation two-photon microscopy.Nat. Methods17, 283-286 (2020)..

Weisenburger, S.et al. Volumetric Ca2+imaging in the mouse brain using hybrid multiplexed sculpted light microscopy.Cell177, 1050-1066.E14 (2019)..

Chakraborty, T. et al. Converting lateral scanning into axial focusing to speed up three-dimensional microscopy.Light.: Sci. Appl.9, 165 (2020)..

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

暂无数据