Parallelized volumetric fluorescence microscopy with a reconfigurable coded incoherent light-sheet array

Yu-Xuan Ren; Jianglai Wu; Queenie T. K. Lai; Hei Ming Lai; Dickson M. D. Siu; Wutian Wu; Kenneth K. Y. Wong; Kevin K. Tsia

doi:10.1038/s41377-020-0245-8

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Parallelized volumetric fluorescence microscopy with a reconfigurable coded incoherent light-sheet array

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- Parallelized volumetric fluorescence microscopy with a reconfigurable coded incoherent light-sheet array
- Parallelized volumetric fluorescence microscopy with a reconfigurable coded incoherent light-sheet array
- LSA 2020年9卷第1期页码：71-81
- Affiliations：
  
  1.Department of Electrical and Electronic Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR 999077, China
  2.Department of Physics, University of California, Berkeley, CA 94720, USA
  3.School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR 999077, China
  4.Department of Psychiatry, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR 999077, China
  5.GHM Institute of CNS Regeneration, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China
  6.Re-Stem Biotechnology, Suzhou 215007, China
- Author bio：
  
  Kevin K. Tsia (tsia@hku.hk)
- Funds：
- DOI：10.1038/s41377-020-0245-8
  中图分类号：
- 纸质出版日期：2020，
  
  网络出版日期：2020-01-20，
  
  收稿日期：2019-05-31，
  
  修回日期：2019-12-23，
  
  录用日期：2020-01-06
- Accepted：
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Parallelized volumetric fluorescence microscopy with a reconfigurable coded incoherent light-sheet array[J]. LSA, 2020,9(1):71-81.

Ren, X. Y. et al. Parallelized volumetric fluorescence microscopy with a reconfigurable coded incoherent light-sheet array. Light: Science & Applications, 9, 71–81 (2020).
Parallelized volumetric fluorescence microscopy with a reconfigurable coded incoherent light-sheet array[J]. LSA, 2020,9(1):71-81. DOI： 10.1038/s41377-020-0245-8.

Ren, X. Y. et al. Parallelized volumetric fluorescence microscopy with a reconfigurable coded incoherent light-sheet array. Light: Science & Applications, 9, 71–81 (2020). DOI： 10.1038/s41377-020-0245-8.

摘要

Abstract

Parallelized fluorescence imaging has been a long-standing pursuit that can address the unmet need for a comprehensive three-dimensional (3D) visualization of dynamical biological processes with minimal photodamage. However

the available approaches are limited to incomplete parallelization in only two dimensions or sparse sampling in three dimensions. We hereby develop a novel fluorescence imaging approach

called coded light-sheet array microscopy (CLAM)

which allows complete parallelized 3D imaging without mechanical scanning. Harnessing the concept of an "infinity mirror"

CLAM generates a light-sheet array with controllable sheet density and degree of coherence. Thus

CLAM circumvents the common complications of multiple coherent light-sheet generation in terms of dedicated wavefront engineering and mechanical dithering/scanning. Moreover

the encoding of multiplexed optical sections in CLAM allows the synchronous capture of all sectioned images within the imaged volume. We demonstrate the utility of CLAM in different imaging scenarios

including a light-scattering medium

an optically cleared tissue

and microparticles in fluidic flow. CLAM can maximize the signal-to-noise ratio and the spatial duty cycle

and also provides a further reduction in photobleaching compared to the major scanning-based 3D imaging systems. The flexible implementation of CLAM regarding both hardware and software ensures compatibility with any light-sheet imaging modality and could thus be instrumental in a multitude of areas in biological research.

关键词

Keywords

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