1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain

Jun Zhu; Hercules Rezende Freitas; Izumi Maezawa; Lee-way Jin; Vivek J. Srinivasan

doi:10.1038/s41377-021-00586-7

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1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain

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- 1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain
- 1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain
- LSA 2021年10卷第8期页码：1507-1519
- Affiliations：
  
  1.Department of Biomedical Engineering, University of California Davis, Davis, CA, 95616, USA
  2.Department of Pathology and Laboratory Medicine, University of California Davis Medical Center, Sacramento, CA, 95817, USA
  3.Department of Ophthalmology and Vision Science, School of Medicine, University of California Davis, Sacramento, CA, 95817, USA
  4.Department of Ophthalmology, NYU Langone Health, New York, NY, 10017, USA
  5.Department of Radiology, NYU Langone Health, New York, NY, 10016, USA
  6.Tech4Health Institute, NYU Langone Health, New York, NY, 10010, USA
- Author bio：
  
  Vivek J. Srinivasan (vjsriniv@ucdavis.edu)
- Funds：
- DOI：10.1038/s41377-021-00586-7
  中图分类号：
- 纸质出版日期：2021-08-31，
  
  网络出版日期：2021-07-14，
  
  收稿日期：2021-02-01，
  
  修回日期：2021-06-25，
  
  录用日期：2021-06-29
- Accepted：
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1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain[J]. LSA, 2021,10(8):1507-1519.

Zhu, J. et al. 1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain. Light: Science & Applications, 10, 1507-1519 (2021).
1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain[J]. LSA, 2021,10(8):1507-1519. DOI： 10.1038/s41377-021-00586-7.

Zhu, J. et al. 1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain. Light: Science & Applications, 10, 1507-1519 (2021). DOI： 10.1038/s41377-021-00586-7.

摘要

Abstract

In vivo

minimally invasive microscopy in deep cortical and sub-cortical regions of the mouse brain has been challenging. To address this challenge

we present an in vivo high numerical aperture optical coherence microscopy (OCM) approach that fully utilizes the water absorption window around 1700 nm

where ballistic attenuation in the brain is minimized. Key issues

including detector noise

excess light source noise

chromatic dispersion

and the resolution-speckle tradeoff

are analyzed and optimized. Imaging through a thinned-skull preparation that preserves intracranial space

we present volumetric imaging of cytoarchitecture and myeloarchitecture across the entire depth of the mouse neocortex

and some sub-cortical regions. In an Alzheimer's disease model

we report that findings in superficial and deep cortical layers diverge

highlighting the importance of deep optical biopsy. Compared to other microscopic techniques

our 1700 nm OCM approach achieves a unique combination of intrinsic contrast

minimal invasiveness

and high resolution for deep brain imaging.

关键词

Keywords

references

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