A silent two-photon imaging system for studying in vivo auditory neuronal functions

Xindong Song; Yueqi Guo; Chenggang Chen; Xiaoqin Wang

doi:10.1038/s41377-022-00783-y

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A silent two-photon imaging system for studying in vivo auditory neuronal functions

Articles

- A silent two-photon imaging system for studying in vivo auditory neuronal functions
- A silent two-photon imaging system for studying in vivo auditory neuronal functions
- LSA 2022年11卷第5期页码：888-900
- Affiliations：
  
  Laboratory of Auditory Neurophysiology, Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
- Author bio：
  
  Xindong Song (songxindong@jhmi.edu)
  Xiaoqin Wang (xiaoqin.wang@jhu.edu)
- Funds：
- DOI：10.1038/s41377-022-00783-y
  中图分类号：
- 纸质出版日期：2022-05-31，
  
  网络出版日期：2022-04-14，
  
  收稿日期：2021-10-25，
  
  修回日期：2022-03-28，
  
  录用日期：2022-03-30
- Accepted：
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A silent two-photon imaging system for studying in vivo auditory neuronal functions[J]. LSA, 2022,11(5):888-900.

Song, X. D. et al. A silent two-photon imaging system for studying in vivo auditory neuronal functions. Light: Science & Applications, 11, 888-900 (2022).
A silent two-photon imaging system for studying in vivo auditory neuronal functions[J]. LSA, 2022,11(5):888-900. DOI： 10.1038/s41377-022-00783-y.

Song, X. D. et al. A silent two-photon imaging system for studying in vivo auditory neuronal functions. Light: Science & Applications, 11, 888-900 (2022). DOI： 10.1038/s41377-022-00783-y.

摘要

Abstract

Two-photon laser-scanning microscopy has become an essential tool for imaging neuronal functions in vivo and has been applied to different parts of the neural system

including the auditory system. However

many components of a two-photon microscope

such as galvanometer-based laser scanners

generate mechanical vibrations and thus acoustic artifacts

making it difficult to interpret auditory responses from recorded neurons. Here

we report the development of a silent two-photon imaging system and its applications in the common marmoset (

Callithrix Jacchus

)

a non-human primate species sharing a similar hearing range with humans. By utilizing an orthogonal pair of acousto-optical deflectors (AODs)

full-frame raster scanning at video rate was achieved without introducing mechanical vibrations. Imaging depth can be optically controlled by adjusting the chirping speed on the AODs without any mechanical motion along the Z-axis. Furthermore

all other sound-generating components of the system were acoustically isolated

leaving the noise floor of the working system below the marmoset's hearing threshold. Imaging with the system in awake marmosets revealed many auditory cortex neurons that exhibited maximal responses at low sound levels

which were not possible to study using traditional two-photon imaging systems. This is the first demonstration of a silent two-photon imaging system that is capable of imaging auditory neuronal functi

ons in vivo without acoustic artifacts. This capacity opens new opportunities for a better understanding of auditory functions in the brain and helps isolate animal behavior from microscope-generated acoustic interference.

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references

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