Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy

Xiaoyi Zhu; Qiang Huang; Anthony DiSpirito; Tri Vu; Qiangzhou Rong; Xiaorui Peng; Huaxin Sheng; Xiling Shen; Qifa Zhou; Laiming Jiang; Ulrike Hoffmann; Junjie Yao

doi:10.1038/s41377-022-00836-2

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Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy

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- Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy
- Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy
- LSA 2022年11卷第6期页码：1238-1252
- Affiliations：
  
  1.Department of Biomedical Engineering, Duke University, Durham, NC, 27708, USA
  2.Department of Pediatric Surgery, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China
  3.Roski Eye Institute, Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, CA, 90033, USA
  4.Department of Biomedical Engineering, University of Southern California, Los Angeles, CA, 90089, USA
  5.Department of Anesthesiology, Duke University, Durham, NC, 27708, USA
- Author bio：
  
  Laiming Jiang (laiming_jiang@foxmail.com)
  Ulrike Hoffmann (ulrike.hoffmann@duke.edu)
  Junjie Yao (junjie.yao@duke.edu)
- Funds：
- DOI：10.1038/s41377-022-00836-2
  中图分类号：
- 纸质出版日期：2022-06-30，
  
  网络出版日期：2022-05-17，
  
  收稿日期：2022-01-04，
  
  修回日期：2022-04-27，
  
  录用日期：2022-05-04
- Accepted：
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Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy[J]. LSA, 2022,11(6):1238-1252.

Zhu, X. Y. et al. Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy. Light: Science & Applications, 11, 1238-1252 (2022).
Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy[J]. LSA, 2022,11(6):1238-1252. DOI： 10.1038/s41377-022-00836-2.

Zhu, X. Y. et al. Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy. Light: Science & Applications, 11, 1238-1252 (2022). DOI： 10.1038/s41377-022-00836-2.

摘要

Abstract

High-speed high-resolution imaging of the whole-brain hemodynamics is critically important to facilitating neurovascular research. High imaging speed and image quality are crucial to visualizing real-time hemodynamics in complex brain vascular networks

and tracking fast pathophysiological activities at the microvessel level

which will enable advances in current queries in neurovascular and brain metabolism research

including stroke

dementia

and acute brain injury. Further

real-time imaging of oxygen saturation of hemoglobin (sO

) can capture fast-p

aced oxygen delivery dynamics

which is needed to solve pertinent questions in these fields and beyond. Here

we present a novel ultrafast functional photoacoustic microscopy (UFF-PAM) to image the whole-brain hemodynamics and oxygenation. UFF-PAM takes advantage of several key engineering innovations

including stimulated Raman scattering (SRS) based dual-wavelength laser excitation

water-immersible 12-facet-polygon scanner

high-sensitivity ultrasound transducer

and deep-learning-based image upsampling. A volumetric imaging rate of 2 Hz has been achieved over a field of view (FOV) of 11 × 7.5 × 1.5 mm

with a high spatial resolution of ~10 μm. Using the UFF-PAM system

we have demonstrated proof-of-concept studies on the mouse brains in response to systemic hypoxia

sodium nitroprusside

and stroke. We observed the mouse brain's fast morphological and functional changes over the entire cortex

including vasoconstriction

vasodilation

and deoxygenation. More interestingly

for the first time

with the whole-brain FOV and micro-vessel resolution

we captured the vasoconstriction and hypoxia simultaneously in the spreading depolarization (SD) wave. We expect the new imaging technology will provide a great potential for fundamental brain research under various pathological and physiological conditions.

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