Upconversion FRET quantitation: the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses

Agata M. Kotulska; Aleksandra Pilch-Wróbel; Satu Lahtinen; Tero Soukka; Artur Bednarkiewicz

doi:10.1038/s41377-022-00946-x

Go to Nature Website

Your Location：

Home >

Browse articles >

Upconversion FRET quantitation: the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses

Original Articles

- Upconversion FRET quantitation: the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses
- Upconversion FRET quantitation: the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses
- LSA 2022年11卷第10期页码：2321-2334
- Affiliations：
  
  1.Division of Biomedical Physicochemistry, Institute of Low Temperature and Structure Research, PAN, ul. Okolna 2, Wrocław 50-422, Poland
  2.Department of Life Technologies/Biotechnology, University of Turku, Kiinamyllynkatu 10, 20520 Turku, Finland
- Author bio：
  
  Tero Soukka (tejoso@utu.fi)
  Artur Bednarkiewicz (a.bednarkiewicz@intibs.pl)
- Funds：
- DOI：10.1038/s41377-022-00946-x
  中图分类号：
- 纸质出版日期：2022-10-31，
  
  网络出版日期：2022-08-19，
  
  收稿日期：2022-02-28，
  
  修回日期：2022-07-03，
  
  录用日期：2022-07-25
- Accepted：
Scan QR Code
Upconversion FRET quantitation: the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses[J]. LSA, 2022,11(10):2321-2334.

Kotulska, A. M. et al. Upconversion FRET quantitation: the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses. Light: Science & Applications, 11, 2321-2334 (2022).
Upconversion FRET quantitation: the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses[J]. LSA, 2022,11(10):2321-2334. DOI： 10.1038/s41377-022-00946-x.

Kotulska, A. M. et al. Upconversion FRET quantitation: the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses. Light: Science & Applications, 11, 2321-2334 (2022). DOI： 10.1038/s41377-022-00946-x.

摘要

Abstract

Lanthanide-doped colloidal nanoparticles capable of photon upconversion (UC) offer long luminescence lifetimes

narrowband absorption and emission spectra

and efficient anti-Stokes emission. These features are highly advantageous for Förster Resonance Energy Transfer (FRET) based detection. Upconverting nanoparticles (UCNPs) as donors may solve the existing problems of molecular FRET systems

such as photobleaching and limitations in quantitative analysis

but these new labels also bring new challenges. Here we have studied the impact of the core-shell compositional architecture of upconverting nanoparticle donors and the mode of photoexcitation on the performance of UC-FRET from UCNPs to Rose Bengal (RB) molecular acceptor. We have quantitatively compared luminescence rise and decay kinetics of Er

emission using core-only NaYF

: 20% Yb

2% Er and core-shell NaYF

: 20% Yb @ NaYF

: 20% Yb

5% Er donor UCNPs under three photoexcitation schemes: (1) direct short-pulse photoexcitation of Er

at 520 nm; indirect photoexcitation of Er

through Yb

sensitizer with (2) 980 nm short (5–7 ns) or (3) 980 nm long (4 ms) laser pulses. The donor luminescence kinetics and steady-state emission spectra differed between the UCNP architectures and excitation schemes. Aiming for highly sensitive kinetic upconversion FRET-based biomolecular assays

the experimental results underline the complexity of the excitation and energy-migration mechanisms affecting the Er

donor responses and suggest ways to optimize the photoexcitation scheme and the architecture of the UCNPs used as luminescent donors.

关键词

Keywords

references

E. Ergin, 等. Time-resolved fluorescence resonance energy transfer [TR-FRET] assays for biochemical processes. Curr. Pharm. Biotechnol., 2016. 171222-1230. .

G. Y. Chen, 等. Upconversion nanoparticles: design, nanochemistry, and applications in theranostics. Chem. Rev., 2014. 1145161-5214. DOI:10.1021/cr400425hhttp://doi.org/10.1021/cr400425h.

X. Qiu, N. Hildebrandt. A clinical role for Förster resonance energy transfer in molecular diagnostics of disease. Expert Rev. Mol. Diagn., 2019. 19DOI:10.1080/14737159.2019.1649144http://doi.org/10.1080/14737159.2019.1649144.

B. Gu, Q. C. Zhang. Recent advances on functionalized upconversion nanoparticles for detection of small molecules and ions in biosystems. Adv. Sci., 2018. 51700609DOI:10.1002/advs.201700609http://doi.org/10.1002/advs.201700609.

M. Safdar, 等. Lanthanide-based inorganic-organic hybrid materials for photon-upconversion. J. Mater. Chem. C., 2020. 86946-6965. DOI:10.1039/D0TC01216Ehttp://doi.org/10.1039/D0TC01216E.

W. R. Algar, 等. FRET as a biomolecular research tool — understanding its potential while avoiding pitfalls. Nat. Methods, 2019. 16815-829. DOI:10.1038/s41592-019-0530-8http://doi.org/10.1038/s41592-019-0530-8.

F. Auzel. Upconversion and anti-stokes processes with f and d ions in solids. Chem. Rev., 2004. 104139-174. DOI:10.1021/cr020357ghttp://doi.org/10.1021/cr020357g.

D. Geißler, 等. Lanthanides and quantum dots as Förster resonance energy transfer agents for diagnostics and cellular imaging. Inorg. Chem., 2014. 531824-1838. DOI:10.1021/ic4017883http://doi.org/10.1021/ic4017883.

N. Sirkka, 等. Upconverting nanophosphors as reporters in a highly sensitive heterogeneous immunoassay for cardiac troponin I. Analytica Chim. Acta, 2016. 92582-87. DOI:10.1016/j.aca.2016.04.027http://doi.org/10.1016/j.aca.2016.04.027.

S. Bhuckory, 等. Core or shell? Er3+ FRET donors in upconversion nanoparticles. Eur. J. Inorg. Chem., 2017. 20175186-5195. DOI:10.1002/ejic.201700904http://doi.org/10.1002/ejic.201700904.

F. Gao, 等. A DNA hybridization detection based on fluorescence resonance energy transfer between dye-doped core-shell silicananoparticles and gold nanoparticles. Analyst, 2011. 1363973-3980. DOI:10.1039/c1an15287dhttp://doi.org/10.1039/c1an15287d.

R. Arppe, 等. Homogeneous assay for whole blood folate using photon upconversion. Anal. Chem., 2015. 871782-1788. DOI:10.1021/ac503691mhttp://doi.org/10.1021/ac503691m.

S. Saraheimo, 等. Time-resolved FRET -based approach for antibody detection - a new serodiagnostic concept. PLoS One, 2013. 8e62739DOI:10.1371/journal.pone.0062739http://doi.org/10.1371/journal.pone.0062739.

A. Teitelboim, 等. Energy transfer networks within upconverting nanoparticles are complex systems with collective, robust, and history-dependent dynamics. J. Phys. Chem. C., 2019. 1232678-2689. DOI:10.1021/acs.jpcc.9b00161http://doi.org/10.1021/acs.jpcc.9b00161.

Y. P. Song, 等. Energy migration control of multimodal emissions in an Er3+ ‐doped nanostructure for information encryption and deep‐learning decoding. Angew. Chem. Int. Ed., 2021. 6023790-23796. DOI:10.1002/anie.202109532http://doi.org/10.1002/anie.202109532.

S. T. Dibaba, 等. Recent progress of energy transfer and luminescence intensity boosting mechanism in Nd3+-sensitized upconversion nanoparticles. J. Rare Earths, 2019. 37791-805. DOI:10.1016/j.jre.2019.02.001http://doi.org/10.1016/j.jre.2019.02.001.

A. Pilch- Wrobel, 等. Engineering the compositional architecture of core‐shell upconverting lanthanide‐doped nanoparticles for optimal luminescent donor in resonance energy transfer: the effects of energy migration and storage. Small, 2022. 182200464DOI:10.1002/smll.202200464http://doi.org/10.1002/smll.202200464.

M. Kaiser, 等. Power-dependent upconversion quantum yield of NaYF4: Yb3+, Er3+ nano- and micrometer-sized particles – measurements and simulations. Nanoscale, 2017. 910051-10058. DOI:10.1039/C7NR02449Ehttp://doi.org/10.1039/C7NR02449E.

A. Pilch, 等. Shaping luminescent properties of Yb3+ and Ho3+ Co-doped upconverting core–shell β-NaYF4 nanoparticles by dopant distribution and spacing. Small, 2017. 131701635DOI:10.1002/smll.201701635http://doi.org/10.1002/smll.201701635.

R. R. Deng, 等. Enabling Förster resonance energy transfer from large nanocrystals through energy migration. J. Am. Chem. Soc., 2016. 13815972-15979. DOI:10.1021/jacs.6b09349http://doi.org/10.1021/jacs.6b09349.

D. Kang, 等. An Efficient NIR-to-NIR signal-based LRET system for homogeneous competitive immunoassay. Biosens. Bioelectron., 2020. 150111921DOI:10.1016/j.bios.2019.111921http://doi.org/10.1016/j.bios.2019.111921.

Meyer, C. et al. Core/shell nanoparticles suitable for (f)ret-assays. (2004).

C. Siefe, 等. Sub-20 Nm core–shell–shell nanoparticles for bright upconversion and enhanced Förster resonant energy transfer. J. Am. Chem. Soc., 2019. 14116997-17005. DOI:10.1021/jacs.9b09571http://doi.org/10.1021/jacs.9b09571.

J. Zhou, 等. Single-molecule photoreaction quantitation through intraparticle-surface energy transfer (i-SET) spectroscopy. Nat. Commun., 2020. 114297DOI:10.1038/s41467-020-18223-zhttp://doi.org/10.1038/s41467-020-18223-z.

A. Das, 等. Quantitative modeling and experimental verification of Förster resonant energy transfer in upconversion nanoparticle biosensors. J. Appl. Phys., 2021. 130023102DOI:10.1063/5.0053464http://doi.org/10.1063/5.0053464.

S. Melle, 等. Förster resonance energy transfer distance dependence from upconverting nanoparticles to quantum dots. J. Phys. Chem. C., 2018. 12218751-18758. DOI:10.1021/acs.jpcc.8b04908http://doi.org/10.1021/acs.jpcc.8b04908.

F. E. Auzel. Materials and devices using double-pumped-phosphors with energy transfer. Proc. IEEE, 1973. 61758-786. DOI:10.1109/PROC.1973.9155http://doi.org/10.1109/PROC.1973.9155.

Wright, J. C. in Radiationless Processes in Molecules and Condensed Phases Ch. 4 (Springer, 2007).

J. S. Kang, 等. Enhanced emission induced by FRET from a long-lifetime, low quantum yield donor to a long-wavelength, high quantum yield acceptor. J. Fluorescence, 2002. 1297-103. DOI:10.1023/A:1015375622992http://doi.org/10.1023/A:1015375622992.

D. N. Xu, 等. Aggregation of rose bengal molecules in solution. J. Photochem. Photobiol. A Chem., 1987. 40DOI:10.1016/1010-6030(87)85013-Xhttp://doi.org/10.1016/1010-6030(87)85013-X.

B. Mendes, 等. Influence of rose bengal dimerization on photosensitization. Photochem. Photobiol., 2021. 97718-726. DOI:10.1111/php.13379http://doi.org/10.1111/php.13379.

V. Muhr, 等. Particle-size-dependent Förster resonance energy transfer from upconversion nanoparticles to organic dyes. Anal. Chem., 2017. 894868-4874. DOI:10.1021/acs.analchem.6b04662http://doi.org/10.1021/acs.analchem.6b04662.

C. Würth, 等. Quantum yields, surface quenching, and passivation efficiency for ultrasmall core/shell upconverting nanoparticles. J. Am. Chem. Soc., 2018. 1404922-4928. DOI:10.1021/jacs.8b01458http://doi.org/10.1021/jacs.8b01458.

S. Y. Han, 等. Lanthanide-doped inorganic nanoparticles turn molecular triplet excitons bright. Nature, 2020. 587594-599. DOI:10.1038/s41586-020-2932-2http://doi.org/10.1038/s41586-020-2932-2.

D. Nettels, 等. Excited-state annihilation reduces power dependence of single-molecule FRET experiments. Phys. Chem. Chem. Phys., 2015. 1732304-32315. DOI:10.1039/C5CP05321Hhttp://doi.org/10.1039/C5CP05321H.

K. Huang, 等. A protected excitation-energy reservoir for efficient upconversion luminescence. Nanoscale, 2018. 10250-259. DOI:10.1039/C7NR06900Fhttp://doi.org/10.1039/C7NR06900F.

A. Baride, 等. Cross-relaxation from Er3+(2H11/2, 4S3/2) and Er3+(2H9/2) in β-NaYF4: Yb, Er and implications for modeling upconversion dynamics. J. Phys. Chem. C., 2020. 1242193-2201. DOI:10.1021/acs.jpcc.9b10163http://doi.org/10.1021/acs.jpcc.9b10163.

V. L. Ermolaev, 等. Co-luminescence of ions and molecules in nanoparticles of metal complexes. Russian Chem. Rev., 2012. 81769-789. DOI:10.1070/RC2012v081n09ABEH004259http://doi.org/10.1070/RC2012v081n09ABEH004259.

J. Y. Fan, 等. (INVITED) Opposing effects of energy migration and cross-relaxation on surface sensitivity of lanthanide-doped nanocrystals. Opt. Mater. X, 2021. 12100104.

Y. H. Li, 等. Investigations on average fluorescence lifetimes for visualizing multi-exponential decays. Front. Phys., 2020. 8576862DOI:10.3389/fphy.2020.576862http://doi.org/10.3389/fphy.2020.576862.

R. B. Anderson, 等. Revisiting the NIR-to-visible upconversion mechanism in β-NaYF4: Yb3+, Er3+. J. Phys. Chem. Lett., 2014. 536-42. DOI:10.1021/jz402366rhttp://doi.org/10.1021/jz402366r.

P. S. May, 等. Tutorial on the acquisition, analysis, and interpretation of upconversion luminescence data. Methods Appl. Fluorescence, 2019. 7023001DOI:10.1088/2050-6120/ab02c6http://doi.org/10.1088/2050-6120/ab02c6.

P. S. May, 等. Measuring the internal quantum yield of upconversion luminescence for ytterbium-sensitized upconversion phosphors using the Ytterbium(III) emission as an internal standard. Nanoscale, 2018. 1017212-17226. DOI:10.1039/C8NR03538Ehttp://doi.org/10.1039/C8NR03538E.

G. Yao, 等. Calculation of judd-ofelt parameters for Er3+ in β-NaYF4: Yb3+, Er3+ from emission intensity ratios and diffuse reflectance spectra. J. Lumin., 2015. 160276-281. DOI:10.1016/j.jlumin.2014.12.025http://doi.org/10.1016/j.jlumin.2014.12.025.

J. Bergstrand, 等. On the decay time of upconversion luminescence. Nanoscale, 2019. 114959-4969. DOI:10.1039/C8NR10332Ahttp://doi.org/10.1039/C8NR10332A.

Hildebrandt, N. in FRET – Förster Resonance Energy Transfer (eds Medintz, I. & Hildebrandt, N. ) Ch. 5 (Wiley-VCH Verlag GmbH & Co. KGaA, 2013).

L. Agazzi, 等. Energy-transfer-upconversion models, their applicability and breakdown in the presence of spectroscopically distinct ion classes: a case study in amorphous Al2O3: Er3+. J. Phys. Chem. C., 2013. 1176759-6776. DOI:10.1021/jp4011839http://doi.org/10.1021/jp4011839.

I. Carrasco, 等. Super-quadratic upconversion luminescence among lanthanide ions. Opt. Express, 2019. 2733217-33232. DOI:10.1364/OE.27.033217http://doi.org/10.1364/OE.27.033217.

L. Ludvíková, 等. Photochemistry of rose bengal in water and acetonitrile: a comprehensive kinetic analysis. Phys. Chem. Chem. Phys., 2016. 1816266-16273. DOI:10.1039/C6CP01710Jhttp://doi.org/10.1039/C6CP01710J.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Light | FRET生物光谱分析技术再获突破

暂无数据