Optical clearing of the mouse skull

Chris Xu

doi:10.1038/s41377-022-00989-0

Go to Nature Website

Your Location：

Home >

Browse articles >

Optical clearing of the mouse skull

News & Views

- Optical clearing of the mouse skull
- Optical clearing of the mouse skull
- LSA 2022年11卷第10期页码：2267-2268
- Affiliations：
  
  School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
- Author bio：
  
  Chris Xu (cx10@cornell.edu)
- Funds：
- DOI：10.1038/s41377-022-00989-0
  中图分类号：
- 纸质出版日期：2022-10-31，
  
  网络出版日期：2022-09-29，
- Accepted：
Scan QR Code
Optical clearing of the mouse skull[J]. LSA, 2022,11(10):2267-2268.

Xu, C. Optical clearing of the mouse skull. Light: Science & Applications, 11, 2267-2268 (2022).
Optical clearing of the mouse skull[J]. LSA, 2022,11(10):2267-2268. DOI： 10.1038/s41377-022-00989-0.

Xu, C. Optical clearing of the mouse skull. Light: Science & Applications, 11, 2267-2268 (2022). DOI： 10.1038/s41377-022-00989-0.

摘要

Abstract

High spatial resolution imaging of the mouse brain through the intact skull is challenging because of the skull-induced aberration and scattering. The research group of Dan Zhu from Huazhong University of Science and Technology has developed a skull-clearing technique that provides a long-term (~ weeks)

stable

transparent window for high resolution optical imaging over a large field of view.

关键词

Keywords

references

G. Yang, F. Pan, C. N. Parkhurst, J. Grutzendler, W.-B. Gan. Thinned-skull cranial window technique for long-term imaging of the cortex in live mice. Nat. Protoc., 2010. 5201-208. DOI:10.1038/nprot.2009.222http://doi.org/10.1038/nprot.2009.222.

P. J. Drew, 等. Chronic optical access through a polished and reinforced thinned skull. Nat. Methods, 2010. 7981-984. DOI:10.1038/nmeth.1530http://doi.org/10.1038/nmeth.1530.

H. T. Xu, F. Pan, G. Yang, W.-B. Gan. The choice of cranial window type for in vivo imaging significantly affects dendritic spine turnover in the cortex. Nat. Neurosci., 2007. 10549-551. DOI:10.1038/nn1883http://doi.org/10.1038/nn1883.

A. Hernandez, 等. Decoding a perceptual decision process across cortex. Neuron, 2010. 66300-314. DOI:10.1016/j.neuron.2010.03.031http://doi.org/10.1016/j.neuron.2010.03.031.

Z. Guo, 等. Flow of cortical activity underlying a tactile decision in mice. Neuron, 2014. 81179-194. DOI:10.1016/j.neuron.2013.10.020http://doi.org/10.1016/j.neuron.2013.10.020.

T. Wang, 等. Three-photon imaging of mouse brain structure and function through the intact skull. Nat. Methods, 2018. 15789-792. DOI:10.1038/s41592-018-0115-yhttp://doi.org/10.1038/s41592-018-0115-y.

T. Wang, C. Xu. Three-photon neuronal imaging in deep mouse brain. Optica, 2020. 7947DOI:10.1364/OPTICA.395825http://doi.org/10.1364/OPTICA.395825.

Qin, Z. et al. Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01343-whttps://doi.org/10.1038/s41587-022-01343-w (2022).

D. G. Ouzounov, 等. In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain. Nat. Methods, 2017. 14388-390. DOI:10.1038/nmeth.4183http://doi.org/10.1038/nmeth.4183.

H. Liu, 等. In vivo deep-brain structural and hemodynamic multiphoton microscopy enabled by quantum dots. Nano Lett., 2019. 195260-5265. DOI:10.1021/acs.nanolett.9b01708http://doi.org/10.1021/acs.nanolett.9b01708.

D. Li, 等. A Through-Intact-Skull (TIS) chronic window technique for cortical structure and function observation in mice. eLight, 2022. 215DOI:10.1186/s43593-022-00022-2http://doi.org/10.1186/s43593-022-00022-2.

J. Wang, Y. Zhang, T. H. Xu, Q. M. Luo, D. Zhu. An innovative transparent cranial window based on skull optical clearing. Laser Phys. Lett., 2012. 9469-473. DOI:10.7452/lapl.201210017http://doi.org/10.7452/lapl.201210017.

C. Zhang, 等. A large, switchable optical clearing skull window for cerebrovascular imaging. Theranostics, 2018. 82696-2708. DOI:10.7150/thno.23686http://doi.org/10.7150/thno.23686.

D.-Y. Li, 等. Visible-near infrared-II skull optical clearing window for in vivo cortical vasculature imaging and targeted manipulation. J. Biophotonics, 2020. 13e202000142DOI:10.1002/jbio.202000142http://doi.org/10.1002/jbio.202000142.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

暂无数据