Parameter estimation of the structured illumination pattern based on principal component analysis (PCA): PCA-SIM

Xin Chen; Yiwei Hou; Peng Xi

doi:10.1038/s41377-022-01043-9

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Parameter estimation of the structured illumination pattern based on principal component analysis (PCA): PCA-SIM

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- Parameter estimation of the structured illumination pattern based on principal component analysis (PCA): PCA-SIM
- Parameter estimation of the structured illumination pattern based on principal component analysis (PCA): PCA-SIM
- 光：科学与应用 2023年12卷第2期页码：150-152
- Affiliations：
  
  1.Department of Biomedical Engineering, College of Future Technology, Peking University, 100871, Beijing, China
  2.National Biomedical Imaging Center, Peking University, 100871, Beijing, China
- Author bio：
  
  Peng Xi (xipeng@pku.edu.cn)
- Funds：
- DOI：10.1038/s41377-022-01043-9
  中图分类号：
- 纸质出版日期：2023-2-28，
  
  网络出版日期：2023-2-8，
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Parameter estimation of the structured illumination pattern based on principal component analysis (PCA): PCA-SIM[J]. 光：科学与应用, 2023,12(2):150-152.

Chen, X., Hou, Y. W. & Xi, P. Parameter estimation of the structured illumination pattern based on principal component analysis (PCA): PCA-SIM. Light: Science & Applications, 12, 150-152 (2023).
Parameter estimation of the structured illumination pattern based on principal component analysis (PCA): PCA-SIM[J]. 光：科学与应用, 2023,12(2):150-152. DOI： 10.1038/s41377-022-01043-9.

Chen, X., Hou, Y. W. & Xi, P. Parameter estimation of the structured illumination pattern based on principal component analysis (PCA): PCA-SIM. Light: Science & Applications, 12, 150-152 (2023). DOI： 10.1038/s41377-022-01043-9.

摘要

Abstract

Principal component analysis (PCA)

a common dimensionality reduction method

is introduced into SIM to identify the frequency vectors and pattern phases of the illumination pattern with precise subpixel accuracy

fast speed

and noise-robustness

which is promising for real-time and long-term live-cell imaging.

关键词

Keywords

references

M. G. Gustafsson . Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy . J. Microsc. , 2000 . 198 82 -87 . DOI:10.1046/j.1365-2818.2000.00710.xhttp://doi.org/10.1046/j.1365-2818.2000.00710.x .

M. G. L. Gustafsson , 等 . Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination . Biophys. J. , 2008 . 94 4957 -4970 . DOI:10.1529/biophysj.107.120345http://doi.org/10.1529/biophysj.107.120345 .

P. Kner , B. B. Chhun , E. R. Griffis , L. Winoto , M. G. L. Gustafsson . Super-resolution video microscopy of live cells by structured illumination . Nat. Methods , 2009 . 6 339 -342 . DOI:10.1038/nmeth.1324http://doi.org/10.1038/nmeth.1324 .

A. Lal , C. Shan , P. Xi . Structured illumination microscopy image reconstruction algorithm . IEEE J. Sel. Top. Quant. , 2016 . 22 50 -63 . DOI:10.1109/JSTQE.2016.2521542http://doi.org/10.1109/JSTQE.2016.2521542 .

G. Wen , 等 . HiFi-SIM: reconstructing high-fidelity structured illumination microscope images . Light Sci. Appl. , 2021 . 10 70 DOI:10.1038/s41377-021-00513-whttp://doi.org/10.1038/s41377-021-00513-w .

J. Demmerle , 等 . Strategic and practical guidelines for successful structured illumination microscopy . Nat. Protoc. , 2017 . 12 988 -1010 . DOI:10.1038/nprot.2017.019http://doi.org/10.1038/nprot.2017.019 .

M. Müller , V. Mönkemöller , S. Hennig , W. Hübner , T. Huser . Open-source image reconstruction of super-resolution structured illumination microscopy data in ImageJ . Nat. Commun. , 2016 . 7 10980 DOI:10.1038/ncomms10980http://doi.org/10.1038/ncomms10980 .

L. M. Hirvonen , K. Wicker , O. Mandula , R. Heintzmann . Structured illumination microscopy of a living cell . Eur. Biophys. J. , 2009 . 38 807 -812 . DOI:10.1007/s00249-009-0501-6http://doi.org/10.1007/s00249-009-0501-6 .

L. Shao , P. Kner , E. H. Rego , M. G. L. Gustafsson . Super-resolution 3D microscopy of live whole cells using structured illumination . Nat. Methods , 2011 . 8 1044 -1046 . DOI:10.1038/nmeth.1734http://doi.org/10.1038/nmeth.1734 .

M. Li , 等 . Structured illumination microscopy using digital micro-mirror device and coherent light source . Appl. Phys. Lett. , 2020 . 116 233702 DOI:10.1063/5.0008264http://doi.org/10.1063/5.0008264 .

A. G. York , 等 . Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy . Nat. Methods , 2012 . 9 749 -754 . DOI:10.1038/nmeth.2025http://doi.org/10.1038/nmeth.2025 .

K. Wicker , O. Mandula , G. Best , R. Fiolka , R. Heintzmann . Phase optimisation for structured illumination microscopy . Opt. Express , 2013 . 21 2032 -2049 . DOI:10.1364/OE.21.002032http://doi.org/10.1364/OE.21.002032 .

S. A. Shroff , J. R. Fienup , D. R. Williams . Phase-shift estimation in sinusoidally illuminated images for lateral superresolution . J. Opt. Soc. Am. A , 2009 . 26 413 -424 . DOI:10.1364/JOSAA.26.000413http://doi.org/10.1364/JOSAA.26.000413 .

S. A. Shroff , J. R. Fienup , D. R. Williams . Lateral superresolution using a posteriori phase shift estimation for a moving object: experimental results . J. Opt. Soc. Am. A , 2010 . 27 1770 -1782 . DOI:10.1364/JOSAA.27.001770http://doi.org/10.1364/JOSAA.27.001770 .

K. Wicker . Non-iterative determination of pattern phase in structured illumination microscopy using auto-correlations in Fourier space . Opt. Express , 2013 . 21 24692 -24701 . DOI:10.1364/OE.21.024692http://doi.org/10.1364/OE.21.024692 .

T. Zhao , 等 . Multi-color structured illumination microscopy for live cell imaging based on the enhanced image recombination transform algorithm . Biomed. Opt. Express , 2021 . 12 3474 -3484 . DOI:10.1364/BOE.423171http://doi.org/10.1364/BOE.423171 .

X. Zhou , 等 . Image recombination transform algorithm for superresolution structured illumination microscopy . J. Biomed. Opt. , 2016 . 21 96009 DOI:10.1117/1.JBO.21.9.096009http://doi.org/10.1117/1.JBO.21.9.096009 .

J. Qian , 等 . Structured illumination microscopy based on principal component analysis . eLight , 2023 . 3 (4 ):DOI:10.1186/s43593-022-00035-xhttp://doi.org/10.1186/s43593-022-00035-x .

A. Markwirth , 等 . Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction . Nat. Commun. , 2019 . 10 4315 DOI:10.1038/s41467-019-12165-xhttp://doi.org/10.1038/s41467-019-12165-x .

X. Huang , 等 . Fast, long-term, super-resolution imaging with Hessian structured illumination microscopy . Nat. Biotechnol. , 2018 . 36 451 -459 . DOI:10.1038/nbt.4115http://doi.org/10.1038/nbt.4115 .

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