Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy

Jonas Zähringer; Fiona Cole; Johann Bohlen; Florian Steiner; Izabela Kamińska; Philip Tinnefeld

doi:10.1038/s41377-023-01111-8

Go to Nature Website

Your Location：

Home >

Browse articles >

Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy

Original Articles

- Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy
- Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy
- 光：科学与应用 2023年12卷第4期页码：585-592
- Affiliations：
  
  1.Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13 Haus E, 81377 München, Germany
  2.Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland
  3.Present address: Department of Physics, Ludwig-Maximilians-Universität München, Schellingstraße 4, 80799 München, Germany
- Author bio：
  
  Philip Tinnefeld (philip.tinnefeld@cup.uni-muenchen.de)
- Funds：
- DOI：10.1038/s41377-023-01111-8
  中图分类号：
- 纸质出版日期：2023-4-30，
  
  网络出版日期：2023-3-10，
  
  收稿日期：2022-10-10，
  
  修回日期：2023-2-7，
  
  录用日期：2023-2-16
Scan for full text
Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy[J]. 光：科学与应用, 2023,12(4):585-592.

Zähringer, J.et al. Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy.Light: Science & Applications,12, 585-592 (2023).
Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy[J]. 光：科学与应用, 2023,12(4):585-592. DOI： 10.1038/s41377-023-01111-8.

Zähringer, J.et al. Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy.Light: Science & Applications,12, 585-592 (2023). DOI： 10.1038/s41377-023-01111-8.

摘要

Abstract

3D super-resolution microscopy with nanometric resolution is a key to fully complement ultrastructural techniques with fluorescence imaging. Here

we achieve 3D super-resolution by combining the 2D localization of pMINFLUX with the axial information of graphene energy transfer (GET) and the single-molecule switching by DNA-PAINT. We demonstrate <2 nm localization precision in all 3 dimension with axial precision reaching below 0.3 nm. In 3D DNA-PAINT measurements

structural features

i.e.

individual docking strands at distances of 3 nm

are directly resolved on DNA origami structures. pMINFLUX and GET represent a particular synergetic combination for super-resolution imaging near the surface such as for cell adhesion and membrane complexes as the information of each photon is used for both 2D and axial localization information. Furthermore

we introduce local PAINT (L-PAINT)

in which DNA-PAINT imager strands are equipped with an additional binding sequence for local upconcentration improving signal-to-background ratio and imaging speed of local clusters. L-PAINT is demonstrated by imaging a triangular structure with 6 nm side lengths within seconds.

关键词

Keywords

references

Huang, B. et al. Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy.Science319, 810–813 (2008).

Pavani, S. R. et al. Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function.Proc. Natl Acad. Sci. USA106, 2995–2999 (2009).

Huang, F. et al. Ultra-high resolution 3D imaging of whole cells.Cell166, 1028–1040 (2016).

Szalai, A. M. et al. Three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules.Nat. Commun.12, 517 (2021).

Gu, L. S. et al. Molecular-scale axial localization by repetitive optical selective exposure.Nat. Methods18, 369–373 (2021).

Dasgupta, A. et al. Direct supercritical angle localization microscopy for nanometer 3D superresolution.Nat. Commun.12, 1180 (2021).

Klar, T. A. et al. Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission.Proc. Natl Acad. Sci. USA97, 8206–8210 (2000).

Balzarotti, F. et al. Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes.Science355, 606–612 (2017).

Weber, M. et al. MINSTED fluorescence localization and nanoscopy.Nat. Photonics15, 361–366 (2021).

Gwosch, K. C. et al. MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells.Nat. Methods17, 217–224 (2020).

Berndt, M. et al. Axial nanometer distances measured by fluorescence lifetime imaging microscopy.Nano Lett.10, 1497–1500 (2010).

Thiele, J. C. et al. Isotropic three-dimensional dual-color super-resolution microscopy with metal-induced energy transfer.Sci. Adv.8, eabo2506 (2022).

Isbaner, S. et al. Axial colocalization of single molecules with nanometer accuracy using metal-induced energy transfer.Nano Lett.18, 2616–2622 (2018).

Kaminska, I. et al. Distance dependence of single-molecule energy transfer to graphene measured with DNA origami nanopositioners.Nano Lett.19, 4257–4262 (2019).

Ghosh, A. et al. Graphene-based metal-induced energy transfer for sub-nanometre optical localization.Nat. Photonics13, 860–865 (2019).

Kamińska, I. et al. Graphene energy transfer for single-molecule biophysics, biosensing, and super-resolution microscopy.Adv. Mater.33, 2101099 (2021).

Nair, R. R. et al. Fine structure constant defines visual transparency of graphene.Science320, 1308 (2008).

Swathi, R. S. & Sebastian, K. L. Resonance energy transfer from a dye molecule to graphene.J. Chem. Phys.129, 054703 (2008).

Masullo, L. A. et al. Pulsed interleaved MINFLUX.Nano Lett.21, 840–846 (2021).

Barroso, M. M. Graphene boost.Nat. Photonics13, 825–826 (2019).

Rothemund, P. W. K. Folding DNA to create nanoscale shapes and patterns.Nature440, 297–302 (2006).

Jungmann, R. et al. Single-molecule kinetics and super-resolution microscopy by fluorescence imaging of transient binding on DNA origami.Nano Lett.10, 4756–4761 (2010).

Strauss, S. & Jungmann, R. Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT.Nat. Methods17, 789–791 (2020).

Chung, K. K. H. et al. Fluorogenic DNA-PAINT for faster, low-background super-resolution imaging.Nat. Methods19, 554–559 (2022).

Schueder, F. et al. An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions.Nat. Methods16, 1101–1104 (2019).

Masullo, L. A. et al. An alternative to MINFLUX that enables nanometer resolution in a confocal microscope.Light Sci. Appl.11, 199 (2022).

Krause, S. et al. Graphene-on-glass preparation and cleaning methods characterized by single-molecule DNA origami fluorescent probes and raman spectroscopy.ACS Nano15, 6430–6438 (2021).

Scheckenbach, M. et al. Self-regeneration and self-healing in DNA origami nanostructures.Angew. Chem. Int. Ed.60, 4931–4938 (2021).

Stehr, F. et al. Tracking single particles for hours via continuous DNA-mediated fluorophore exchange.Nat. Commun.12, 4432 (2021).

Zhang, Z. Y. et al. Ultrahigh-throughput single-molecule spectroscopy and spectrally resolved super-resolution microscopy.Nat. Methods12, 935–938 (2015).

Fullbrunn, N. et al. Nanoscopic anatomy of dynamic multi-protein complexes at membranes resolved by graphene-induced energy transfer.eLife10, e62501 (2021).

Cordes, T., Vogelsang, J. & Tinnefeld, P. On the mechanism of Trolox as antiblinking and antibleaching reagent.J. Am. Chem. Soc.131, 5018–5019 (2009).

Nickels, P. C. et al. Molecular force spectroscopy with a DNA origami-based nanoscopic force clamp.Science354, 305–307 (2016).

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Light | 新型3D超分辨纳米显微镜

暂无数据