1.School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3AX, UK
2.School of Biochemistry, University of Bristol, University Walk, Bristol, UK
3.School of Physics and Astronomy, Cardiff University, The Parade, Cardiff, CF24 3AA, UK
4.Present address: Department of Chemistry, Umeå University, Umeå, 90187, Sweden
5.Present address: School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK
Paola Borri (BorriP@cardiff.ac.uk)
Paul Verkade (P.Verkade@bristol.ac.uk)
纸质出版日期:2023-4-30,
网络出版日期:2023-3-29,
收稿日期:2022-9-14,
修回日期:2023-2-9,
录用日期:2023-2-21
Scan for full text
Correlative light-electron microscopy using small gold nanoparticles as single probes[J]. 光:科学与应用, 2023,12(4):657-670.
Pope, I.et al. Correlative light-electron microscopy using small gold nanoparticles as single probes.Light: Science & Applications,12, 657-670 (2023).
Correlative light-electron microscopy using small gold nanoparticles as single probes[J]. 光:科学与应用, 2023,12(4):657-670. DOI: 10.1038/s41377-023-01115-4.
Pope, I.et al. Correlative light-electron microscopy using small gold nanoparticles as single probes.Light: Science & Applications,12, 657-670 (2023). DOI: 10.1038/s41377-023-01115-4.
Correlative light-electron microscopy (CLEM) requires the availability of robust probes which are visible both in light and electron microscopy. Here we demonstrate a CLEM approach using small gold nanoparticles as a single probe. Individual gold nanoparticles bound to the epidermal growth factor protein were located with nanometric precision background-free in human cancer cells by light microscopy using resonant four-wave mixing (FWM)
and were correlatively mapped with high accuracy to the corresponding transmission electron microscopy images. We used nanoparticles of 10 nm and 5 nm radius
and show a correlation accuracy below 60 nm over an area larger than 10 µm size
without the need for additional fiducial markers. Correlation accuracy was improved to below 40 nm by reducing systematic errors
while the localisation precision is below 10 nm. Polarisation-resolved FWM correlates with nanoparticle shapes
promising for multiplexing by shape recognition in future applications. Owing to the photostability of gold nanoparticles and the applicability of FWM microscopy to living cells
FWM-CLEM opens up a powerful alternative to fluorescence-based methods.
Ando, T. et al. The 2018 correlative microscopy techniques roadmap.J. Phys. D: Appl. Phys.51, 443001 (2018).
Muller-Reichert, T. & Verkade, P. Correlative light and electron microscopy. In Methods in Cell Biology (Elsevier, Academic Press, Cambridge MA, United States, 2021, Series Editors: Leslie Wilson and Phong Tran).
Brown, E. & Verkade, P. The use of markers for correlative light electron microscopy.Protoplasma244, 91–97 (2010).
Tanner, H. et al. Fluorescent platinum nanoclusters as correlative light electron microscopy probes.Methods Cell Biol.162, 39–68 (2021).
Kandela, I. K. & Albrecht, R. M. Fluorescence quenching by colloidal heavy metals nanoparticles: Implications for correlative fluorescence and electron microscopy studies.Scanning29, 152–161 (2007).
Miles, B. T. et al. Direct evidence of lack of colocalisation of fluorescently labelled gold labels used in correlative light electron microscopy.Sci. Rep.7, 44666 (2017).
Giannakopoulou, N. et al. Four-wave-mixing microscopy reveals non-colocalisation between gold nanoparticles and fluorophore conjugates inside cells.Nanoscale12, 4622–4635 (2020).
Giepmans, B. N. G. et al. Correlated light and electron microscopic imaging of multiple endogenous proteins using quantum dots.Nat. Methods2, 743–749 (2005).
Yuan, G. C. et al. Two mechanisms determine quantum dot blinking.ACS Nano12, 3397–3405 (2018).
Kukulski, W. et al. Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision.J. Cell Biol.192, 111–119 (2011).
Johnson, E. et al. Correlative in-resin super-resolution and electron microscopy using standard fluorescent proteins.Sci. Rep.5, 9583 (2015).
Sartori, A. et al. Correlative microscopy: bridging the gap between fluorescence light microscopy and cryo-electron tomography.J. Struct. Biol.160, 135–145 (2007).
Tuijtel, M. W. et al. Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins.Sci. Rep.9, 1369 (2019).
Hoffman, D. P. et al. Correlative three-dimensional super-resolution and block-face electron microscopy of whole vitreously frozen cells.Science367, eaaz5357 (2020).
Zoriniants, G. et al. Background-free 3D nanometric localization and sub-nm asymmetry detection of single plasmonic nanoparticles by four-wave mixing interferometry with optical vortices.Phys. Rev. X7, 041022 (2017).
Pope, I. et al. Background-free 3D four-wave mixing microscopy of single gold nanoparticles inside biological systems. Proceedings of SPIE-OSA 11922, Advances in Microscopic Imaging III. SPIE, 2021, 119220Q. (Event: European Conferences on Biomedical Optics, 2021, Online Only).
Verkade, P. Moving EM: the rapid transfer system as a new tool for correlative light and electron microscopy and high throughput for high-pressure freezing.J. Microsc.230, 317–328 (2008).
Van Weering, J. R. T. et al. Intracellular membrane traffic at high resolution.Methods Cell Biol.96, 619–648 (2010).
Masia, F., Langbein, W. & Borri, P. Measurement of the dynamics of plasmons inside individual gold nanoparticles using a femtosecond phase-resolved microscope.Phys. Rev. B85, 235403 (2012).
Manzo, C. & Garcia-Parajo, M. F. A review of progress in single particle tracking: from methods to biophysical insights.Rep. Prog. Phys.78, 124601 (2015).
Liu, S. L. et al. Single-virus tracking: from imaging methodologies to virological applications.Chem. Rev.120, 1936–1979 (2020).
Zhang, W. J. et al. Encapsulation of inorganic nanomaterials inside virus-based nanoparticles for bioimaging.Nanotheranostics1, 358–368 (2017).
Masia, F. et al. Resonant four-wave mixing of gold nanoparticles for three-dimensional cell microscopy.Opt. Lett.34, 1816–1818 (2009).
Berciaud, S. et al. Photothermal heterodyne imaging of individual metallic nanoparticles: theory versus experiment.Phys. Rev. B73, 045424 (2006).
Zilli, A., Langbein, W. & Borri, P. Quantitative measurement of the optical cross sections of single nano-objects by correlative transmission and scattering microspectroscopy.ACS Photonics6, 2149–2160 (2019).
Payne, L. M. et al. The optical nanosizer – quantitative size and shape analysis of individual nanoparticles by high-throughput widefield extinction microscopy.Nanoscale12, 16215–16228 (2020).
Hosogi, N., Nishioka, H. & Nakakoshi, M. Evaluation of lanthanide salts as alternative stains to uranyl acetate.Microscopy64, 429–435 (2015).
Ishii, N. Systematic investigation of lanthanoid transition heavy metal acetates as electron staining reagents for protein molecules in biological transmission electron microscopy.Microsc. Microanal.28, 780–789 (2022).
Carnall, W. T., Fields, P. R. & Rajnak, K. Spectral intensities of the trivalent lanthanides and actinides in solution. II. Pm3+, Sm3+, Eu3+, Gd3+, Tb3+, Dy3+, and Ho3+.J. Chem. Phys.49, 4412–4423 (1968).
Takahashi, Y. & Akiyama, H. Heat capacity of gold from 80 to 1000 k.Thermochim. Acta109, 105–109 (1986).
Fahy, G. M. & Wowk, B. Principles of ice-free cryopreservation by vitrification. In: Cryopreservation and Freeze-Drying Protocols (eds. Wolkers, W. F. & Oldenhof, H. ), 27–97 (New York: Springer, 2021).
Wu, P. H. et al. High-throughput ballistic injection nanorheology to measure cell mechanics.Nat. Protoc.7, 155–170 (2012).
Jiang, Z. D. et al. Genetically encoded tags for direct synthesis of EM-visible gold nanoparticles in cells.Nat. Methods17, 937–946 (2020).
Hodgson, L., Verkade, P. & Yamauchi, Y. Correlative light and electron microscopy of influenza virus entry and budding.Methods Mol. Biol.1836, 237–260 (2018).
0
浏览量
0
Downloads
0
CSCD
关联资源
相关文章
相关作者
相关机构
京公网安备11010802024621